Rabbit semen dilution and precautions

The commonly used dilution sodium citrate glucose dilute solution: sodium citrate 0.38 g, anhydrous glucose 4.54 g, egg yolk 1 ~ 3 ml, penicillin, streptomycin each 100,000 international units, distilled water was added to 100 ml. Sucrose yolk diluent: 11 grams of sucrose, 1 to 3 ml of yolk, 100,000 international units of penicillin and streptomycin, and 100 ml of distilled water. Glucose yolk dilution: 7.5 g of anhydrous glucose, 1-3 ml of egg yolk, 100,000 international units of penicillin and streptomycin, and 100 ml of distilled water.

Preparation of diluent should pay attention to the items to clean, dry, prior to disinfection, distilled water, eggs should be fresh, the drugs used should be pure and reliable. Drugs should be weighed accurately. After the drug is dissolved, it is filtered and boiled for 15 to 20 minutes.

Things to be aware of when diluting semen Dilutions and semen should be diluted at the same temperature. Dilutions should be slowly poured along the vessel wall into the container containing the semen. Do not reverse it, otherwise it will affect the sperm's survival. Need high (5 times more) dilution.

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