Filling of the gel filter column
For gel filtration, the choice of column is generally based on the type of sample being separated and the number of samples. When purifying the protein, the volume of the bed should be 25 to 100 times the volume of the sample. The salt removal and free fluorescein are about 4 to 10 times the volume of the sample. The column is too short to affect the separation. The column length is longer and the separation effect is good, but if the column is too long, the separation time is prolonged and the sample is also diluted excessively. The inner diameter of the column should also be chosen appropriately. If the inner diameter is too thin, a "wall effect" will occur, that is, the flow rate near the tube wall is greater than the flow rate at the center to affect the separation effect. Therefore, the inner diameter and height of the column should be proportional. It should be 1..5~1..25 ​​for salt removal and 1..20~1..100 for purified protein.
The filling method and requirements of the gel column are basically the same as those of the ion exchange column. An ideal gel column requires uniform packing (gel) density in the column without voids and bubbles. Usually, the newly installed gel column is equilibrated with a suitable buffer solution, and the colored glucomannan-2000, cytochrome, or hemoglobin is formulated into a solution having a mass concentration of 2 g/L to observe whether the ribbon is uniform. Move down to verify that the technical quality of the new packed column is acceptable, otherwise it must be refilled.
Chinese Gallnut Extract also known as Chinese gall or nutgalls. It is a plant excretion produced when irritants are released by the larvae of gall insects, such as those of the Cynipidae family, the gall wasps. Oak trees are the major commercial source of medicinal gallnuts.
1. With the function of antioxidant and antimicrobial;
2. With the function of treating chronic diarrhea and dysentery
3. With the function of enhancing immunity.
4.It has the effect on improving the body disease-resistant ability, liver and gastrointestinal systemfunction;
5. With the function of dispelling heat, diuretic, eliminating irritability, cooling blood and detoxification;
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