Cat Glutamate (GLU) Kit Kit

Cat Glutamate (GLU) Kit Procedure
1) Mix all reagents thoroughly before use. Do not allow the liquid to generate a large amount of foam, so as to avoid adding a large amount of air bubbles during the loading, resulting in errors in the loading.
2) Determine the number of slats required based on the number of samples to be tested plus the number of standards. Multiple holes are recommended for each standard and blank hole. Each sample is determined according to its own quantity, and the double hole can be used as much as possible. The specimen was diluted 1:1 with the specimen dilution and 50 ul was added to the reaction well.
3) Add 50 ul of the diluted standard to the reaction well, and add 50 ul of the sample to be tested to the reaction well. Immediately add 50 ul of biotinylated antibody. Cover the membrane, mix gently by shaking, and incubate for 1 hour at 37 °C.
4) Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
5) Add 80 ul of affinity streptavidin-HRP per well, mix gently by shaking, and incubate at 37 ° C for 30 minutes.
6) Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
7) Add 50 ul of each of the substrates A and B to each well, mix gently by shaking, and incubate at 37 ° C for 10 minutes. Avoid lighting.
8) Remove the microplate and quickly add 50 ul of stop solution. Immediately after adding the stop solution, the results should be determined.
9) The OD value of each well was measured at a wavelength of 450 nm.

Domestic cat glutamic acid (GLU) kit limitations
The results above the No. 6 standard are non-linear and no accurate results can be obtained from this standard curve.

Domestic Cat Glutamate (GLU) Kit Kit Performance
1. Sensitivity: The minimum detection concentration is less than the No. 1 standard. The linearity of the dilution. The linear regression coefficient of the sample and the expected concentration correlation coefficient R value was 0.990.
2. Specificity: Does not react with other cytokines.
3. Repeatability: The coefficient of variation between the plate and the plate is less than 10%.

Judgment and Analysis of Domestic Cat Glutamate (GLU) Kit Results
1. Instrument value: read the OD value of each hole on a microplate reader with a wavelength of 450 nm.
2. The absorbance OD value is the ordinate (Y), and the corresponding test substance concentration is the abscissa (X), and the corresponding curve is made. The content of the sample to be tested can be converted from the standard curve according to the OD value. The corresponding concentration.

Resist wrinkles

Resist wrinklesProducts,Oligomeric Proantho Cyanidins ,AntiCaprylhydroxamic Acid in Skin,Sepiwhite Msh Powder Factory,Glycyrrhizic Acid Foods,

China Anti-Wrinkle Products,Glutathione Oral Solution manufacturers, welcome Nonapeptide-1 Pharmaceutical Api,Hexapeptide-11 Active Ingredients purchasers from worldwide to visit our site.

Resist wrinklesProducts,Oligomeric Proantho Cyanidins,AntiCaprylhydroxamic Acid in Skin,Sepiwhite Msh Powder Factory,Glycyrrhizic Acid Foods

Xi'an Henrikang Biotech Co.,Ltd , https://www.henruikangbio.com