China's tissue engineering human cornea stage successful corneal patients are expected to regain the bright future
Release date: 2010-11-12
Recently, the tissue engineering human corneal endothelial transplantation experiment developed by the Corneal Tissue Engineering Laboratory of Ocean University of China has achieved great success. This research results not only through the corneal endothelium blind patients and corneal endothelial cell decompensation after cataract surgery through the engineering cornea The re-emergence of endothelial transplants is about to become a reality, and it has also made it possible to successfully reconstruct the entire cornea and to see the blindness of various corneal blind patients.
A few days ago, the reporter learned from China Ocean University that the tissue engineering human corneal endothelial animal transplantation experiment developed by the Corneal Tissue Engineering Laboratory of Ocean University of China has achieved great success. As of July 8, 2010, the transplanted tissue engineered human corneal endothelium has kept the New Zealand rabbit cornea transparent for 391 days, the domestic cat cornea has remained transparent for 209 days, and the macaque has maintained corneal transparency for 119 days, which is the first time in the world.
Director of the Key Laboratory of Corneal Tissue Engineering, Ocean University of China, Director of the National Life Science and Technology Talents Training Base of Ocean University of China, Vice President of the Marine Life College of Ocean University of China, and Fan Tingjun, the leader of the National Cell Biology Teaching Team, said: "The results of this research Not only the patients with corneal endothelium blindness and corneal endothelial cell decompensation after cataract surgery will be a reality through the engineering transplant of corneal endothelium. It will also make the successful reconstruction of the whole cornea and the re-emergence of various corneal blind patients." The pain of corneal blind patients We often say that the eyes are the window of the soul. Through the clear and transparent eyes, we can see the colorful world outside and feel the clear and transparent heart of one person. In the human eye, the cornea can be regarded as one of the most important components. The healthy cornea is completely transparent and has a certain radius of curvature and refractive power. Together with the refractive power of the lens, the light can be accurately focused on the retina of the fundus, forming a beautiful world in front of us.
In the world, nearly half of low-vision patients lose their light because of corneal lesions.
According to the statistics of the World Health Organization, there are currently more than 135 million people with low vision in the world, including about 60 million people with corneal blindness. In China, there are more than 12.3 million patients with low vision, of which about 5 million are caused by corneal diseases. People, there are about 450,000 new blind people in China every year. It is worth mentioning that among the patients with corneal blindness, young adults account for about 70% and children account for about 15%. Not only do they suffer from blindness for a long time, they cannot live, study and work normally, but also bring family and society. A huge spiritual and economic burden. At present, corneal transplantation is the only hope for blindness in patients with corneal blindness. However, due to the extremely limited number of donor corneas, the annual corneal transplantation in the country is not enough for the majority of patients with corneal blindness. The cornea cannot be transplanted and cannot be recovered.
In the above-mentioned corneal blind patients, the corneal endothelium blind patients account for about 1 to 5-1 to 4 because the density of corneal endothelial cells is lower than the critical density for maintaining the physiological function of the corneal endothelium. In addition, there are more than 5 million cataract patients in China, and nearly 30 million people worldwide. Because of the complications of clinical surgery, a large number of corneal endothelial cells die, which eventually causes corneal endothelial cells to be decompensated and gradually become blind. Can not be treated and recovered without transplanting the cornea.
It is precisely because the corneal endothelium plays an irreplaceable role in maintaining corneal thickness, transparency, and feeding and oxygen supply. Whether tissue engineered human corneal endothelium (hereinafter referred to as "engineered corneal endothelium") can be successfully reconstructed in vitro is the decision of engineering full corneal energy. Whether the key to successful in vitro reconstruction.
"Therefore, the reconstruction of the engineered corneal endothelium in vitro can not only make the corneal endothelium blind and cataract patients clear, but also solve the new corneal blind disease to make the corneal blind patients see the new bright hope." Fan Tingjun said. Corneal tissue engineering - the light of re-exposure in patients with corneal blindness The use of tissue engineering techniques to reconstruct the corneal endothelium is the hope of recovery of corneal blind patients, and it is also the key to fundamentally solve the problems of corneal graft donor material deficiency and postoperative immune rejection. Fan Tingjun said that how to obtain a large number of functional human corneal seed cells in vitro and how to develop a biocompatible ideal carrier scaffold is the two key elements for the successful reconstruction of corneal endothelium and engineered whole cornea in vitro. Research hotspots and main targets of corneal tissue engineering.
Because human corneal endothelial cells lose their ability to divide in adulthood, it is the most difficult to culture and establish in vitro. The lack of sufficient human corneal endothelial seed cells has become the main bottleneck for limiting the reconstruction of corneal endothelium and even the whole cornea in vitro. In vitro culture studies of human corneal endothelial cells began in the late 1950s. Since then, many scholars have begun the in vitro culture and establishment of human corneal endothelial cells, and also established several immortalized human corneal endothelial cell lines by oncogene transfection technology, but the immortalized cells obtained by oncogene transfection have The potential tumorigenicity limits its use in engineered corneal endothelium construction, especially in clinical transplantation.
The key to the successful reconstruction of the corneal endothelium and engineered whole cornea in vitro is to use the ideal carrier scaffold to allow the seed cells to grow and proliferate normally and finally form a normal tissue structure similar to the corneal endothelium and the whole cornea. The in vitro reconstruction of tissue engineered artificial corneal endothelium using carrier scaffolds and human corneal endothelial cells began in the early 1890s. The researchers used vector scaffolds and human corneal endothelial cells to reconstruct corneal endothelium analogs with approximate corneal endothelium structure and partial corneal endothelial function, and can maintain corneal transparency for up to a week in animals such as New Zealand rabbits. This has opened the way for the reconstruction of engineered corneal endothelium using vector scaffolds and human corneal endothelial cells.
"But this method still reveals a lot of problems." Fan Tingjun explained that, first of all, the seed cells used by some scholars are immortalized human corneal endothelial cells transfected with oncogenes, which have limited tumorigenicity and thus limit the engineering cornea. The application of the endothelium in its clinical application; secondly, the seed cells used by some scholars are primary cultured cells derived from the cornea of ​​the eye bank or cells of only 4 to 5 passages in the 24-well culture plate, due to the limitation of the number of seed cells. The number of engineered corneal endothelium that can be reconstructed is very limited, which can not meet the needs of clinical treatment of many patients with corneal endothelium blindness. Again, most of the carrier scaffolds currently used are artificial composite materials, and their biocompatibility to human corneal endothelial cells is Not ideal enough, and the impact of its degradation on the human body is still unclear. In addition, the reconstructed engineered corneal endothelium can only keep the corneas of rabbits and other animals transparent for about 1 week, which is far from the clinical transplantation and treatment successfully applied to corneal endothelium.
Since 2002, the Corneal Tissue Engineering Laboratory of Ocean University of China has been funded by the National “10th Five-Year†“863†Project and the “Eleventh Five-Year†and “863†Major Projects for the first time in the world through more than 9 years of painstaking research. The only non-tumorigenic human corneal endothelial cell line that has not been transfected with any oncogene was established, and the commercial amnion treatment and modification technology was established. The human corneal endothelium and modified amniotic membrane were successfully used to establish the engineering corneal endothelium. Through the production technology, the engineered corneal endothelium with a cell density of up to 3400-3600/mm2 was obtained. As of July 8, 2010, the engineered corneal endothelium produced has kept the New Zealand rabbit cornea transparent for 391 days and domestic cats for 209 days. Macaque 119 days. The research results on "in vitro reconstruction of tissue engineered human corneal endothelium and transplantation of New Zealand rabbits" successfully passed the scientific and technological achievements appraisal organized by the Ministry of Education in Beijing in September 2009, and won the "International Innovation" and reached the international leading level. "The conclusion of the appraisal."
At present, under the leadership of Fan Tingjun, the laboratory has completed the production of Class III medical devices in cooperation with Qingdao Yuming Biotechnology Co., Ltd., and obtained the production license for Class III implant materials and artificial organs. The industrial production of corneal endothelium was prepared. At the same time, in cooperation with Qingdao Zhonghao Bioengineering Co., Ltd., the in vitro reconstruction of tissue engineering human cornea was started. The tissue engineering human corneal epithelium has been successfully developed. Within 5 years, the tissue engineered human whole cornea was developed and its animal transplantation experiment was completed. Independent innovation to overcome international problems In the research work of this subject, the Fan Tingjun team of the Corneal Tissue Engineering Laboratory of Ocean University of China has fully exerted the spirit of arduous struggle and independent innovation, especially able to endure hardships, especially to tackle the problem, and achieved many innovations. The world is eye-catching.
Among the many achievements, the most influential is the establishment of the world's only human corneal endothelial cell line that has not been transfected with a cancer gene and has no tumorigenicity, and has selected a normal karyotype. Monoclonal cell line, the first successful solution to the international problem of tissue engineering artificial corneal endothelium production lacking sufficient seed cells; in addition, the project established a commercial amnion treatment and modification technology, and obtained Human corneal endothelial cells have ideal biocompatibility carrier scaffolds, which successfully solved the international technical problem of large-scale carrier scaffolds for tissue engineering artificial corneal endothelium production. In addition, this project has also established human cornea for the first time in the world. The endothelial monoclonal cell strain is a seed cell, and the modified corneal membrane is used as a carrier to produce artificial corneal endothelium in vitro. The artificial corneal endothelium with normal structure and function is obtained. For the first time, the corneal transplantation donor material is lacking and the artificial corneal endothelium is fundamentally solved. An international problem that cannot be scaled up in vitro.
It is worth mentioning that the artificial corneal endothelium produced by the project can maintain the cornea transplanted in New Zealand rabbits for more than 277 days and the domestic cats for more than 93 days. The cornea of ​​the transplanted macaques has also been transparent, and it has been successfully solved for the first time in the world. Artificial corneal endothelium does not provide an international challenge to maintain long-term transparency of the cornea of ​​transplanted animals.
The great success of the corneal tissue engineering project is China's independent intellectual property rights, which has created extremely favorable conditions for China to occupy the international commanding heights of tissue engineering corneal production and its clinical application. Engineering corneal industrialization - creating benefits for the benefit of humans Fan Tingjun further pointed out that the industrialization of engineered corneal endothelium and artificial full cornea can be used for corneal endothelium blindness, corneal blindness and corneal transplantation and treatment of various corneal diseases, so that the majority of corneal blind patients Seeing the light again will not only create huge economic benefits, but also generate huge social benefits.
There are more than 5 million patients with corneal blindness and more than 5 million cataract patients in China, and they are increasing year by year. As the only substitute for corneal endothelium blindness and corneal blind clinical transplantation, the engineered corneal endothelium and artificial total cornea have been used for clinical corneal transplantation and treatment of various corneal endothelial blindness and corneal blindness. This can not only make a large number of the above-mentioned various corneal blind patients see the light again, relieve the suffering and suffering of these patients suffering from blindness for a long time, but also relieve the mental and life pressure brought to the family by the blindness of the patients, and is more conducive to lifting the problem. The enormous economic burden brought to the country and the government is of great significance to the building of a harmonious society in China.
On the other hand, the engineered corneal endothelium and engineered whole cornea produced by the corneal tissue engineering project are also considerable in helping patients to relieve the pain and the economic benefits. In terms of engineering corneal endothelium, the patient's transplant coverage rate is estimated at 70%, and domestic direct economic benefits can be 40 billion to 60 billion yuan, and the world's 300-400 billion yuan; for the whole cornea, the patient's The transplant coverage rate is estimated at 70%, and the direct economic benefits generated by domestic and foreign countries are 40 billion to 50 billion yuan and 400 billion to 600 billion yuan respectively.
The industrialization and clinical application of engineering corneal endothelium products and engineering whole corneal products can not only produce great social benefits, but also produce huge economic benefits. It is a vision that can make blind corneal blind patients all over the world. A bright engineering project that promotes economic and social development.
Source: Science Times
Yunnan NBC Agricultural Development Co., Ltd deals in (heat pump air drying) Yunnan Plateau agricultural products. Including Flowers And Leaves, Fruits, rhizomes, poultry meat, aquatic products and other drying products. All products are Free of Additives, agricultural residues and phosphorus and sulfur. It has obtained the national SC food safety production certification, and at the same time, it is produced according to the international standards, reaching the export level, and can be OEM.
Edible Fungus,Dry Edible Fungus,Dried Edible Fungus,Free of Additives
Yunnan New Biology Culture Co,.Ltd , https://www.lvsancoffee.com