"Control" cell fate with a beam of far red light

"Control" cell fate with a beam of far red light

July 4, 2018 Source: Science and Technology Daily Author: Wang

Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];

July 2nd, US time (July 3, Beijing time), from the School of Life Sciences of East China Normal University, Shanghai Key Laboratory of Regulatory Biology, the research team of "Young Thousand People" Ye Haifeng in the international authoritative magazine "National Academy of Sciences The latest scientific research results were published in the Journal.

The researchers used the multi-disciplinary techniques of synthetic biology, optogenetics, gene editing, and regenerative medicine to develop the transcriptional activation device of CRISPR-dCas9 endogenous gene regulated by far red light. For the first time, the results achieved the use of far red light to manipulate the expression regulation of genomic genes, and established a technical system for the regulation of endogenous gene expression by far red light. In theory, using this technology, as long as a beam of far red light can control the differentiation of stem cells into any desired functional cells, such as heart cells, nerve cells and so on. This technology can be widely used for precise epigenetic regulation or reprogramming, providing strong technical support for the study of endogenous genomic gene function and translational medicine application research.

Ye Haifeng, who is in charge of the research, told reporters that they use the concepts of synthetic biology and optogenetics to derive the protein BphS that responds to far red light in red bacteria, the transcription factor BldD in Streptococcus, and the dCas9 protein in Streptococcus pyogenes. The CRISPR-dCas9 endogenous gene transcription device (referred to as FACE system) regulated by far-red light was artificially designed, spliced ​​and assembled. For the first time, the study achieved the use of far-red light to manipulate the expression of endogenous genes in the genome at the cellular level and in animals, and successfully induced the induced pluripotent stem cells to differentiate into functional nerve cells.

It is reported that researchers using far-red light to regulate this system can achieve reversible activation of target genes in vitro and in vivo through precise regulation of light beams, with high temporal and spatial specificity, strong tissue penetration and non-toxic side effects. In the future, in the field of regenerative medicine, such as the treatment of muscle atrophy, it will bring new hope.

The research further developed the optogenetics toolbox, which laid the foundation for the basic theoretical research and transformation application research of the precise spatiotemporal genetic regulation of mammalian cell genome, and further promoted the precise treatment and clinical transformation research based on optogenetics.

The research work was funded by the National Natural Science Foundation of China, the Ministry of Science and Technology's major stem cell project, the Shanghai Science and Technology Commission, the Youth Thousand Talents Program, and the East China Normal University talent team.

Greenhouse Plastic Plastic Clamps

Greenhouse Plastic Plastic Clamps,Greenhouse Structure Connecting Clamps,Agricultural Greenhouses Clamps,Plastic Clamps

JIANGSU SKYPLAN GREENHOUSE TECHNOLOGY CO.,LTD , https://www.skyplantgreenhouse.com