Establishment of a rat pain model, animal model of Alzhermer's disease, detailed scoring criteria for neurological deficits in rats

Detailed scoring criteria for neurological deficits in rats

1 . Neurobehavioral score

In infarction after 24 h, according to Masao Shmi izu-Sasamata method [3] All rats behavioral neurological scoring criteria include: â‘  degree of locomotor activity, the left forelimb hemiplegia â‘¡, â‘¢ when lifting the tail stretched straight left forelimb 4 anti-roll thrust ability , 5 left-to-left tilt , 6 left-hand loop , 7 pairs of tentacles. The above indicators had no abnormality of 0 points , moderate abnormalities of 1 point , and severe abnormalities of 2 points . The scores were added together and the total score was 0 to 14 points.

2 . Animal behavioral assessment

10 points : no symptoms of nerve damage ;

21 points : the contralateral front paws cannot be fully extended ;

32 points : turn to the side of the squat ;

43 points : dumping to the opposite side ;

54 points : Can not be released from the release , loss of consciousness.

3 . Nerve injury in rats severe impairment score (Neurological Severity Scores, NSS):

0 points : normal nerve function ;

1 point : mild neurological deficit ( buckling of the left forelimb during tailing );

2 points : moderate neurological deficit ( turning to the left while walking );

3 points : moderate neurological deficit ( tilted to the left );

4 points : no self-issuance, loss of consciousness ;

5 points : Death associated with ischemia.

4. Test balance beam (Beam Balance Test, BBT):

The rats were placed on a 1.5 cm wide strip of wood. The wooden strip was suspended at one end and the other end was fixed to the center of a 40x40 cm flat plate to prevent the rat from climbing from the wooden strip to the table to make the experiment fail. A cushion is placed under the wooden strip to prevent the rat from falling when it falls. According to balance of rats within two minutes neurological score line. The balance ability of normal rats is 1-2 minutes.

Balance test score criteria:

1 Stand firm on the wooden strip without shaking for 2 minutes

2 Stand firmly on the wooden strip, shake it from side to side, not slipping down for 2 minutes

3 Stand on the wooden strip, slide down to one side, not falling down for 2 minutes

4When standing on the wooden strip, it will fall off the wooden strip in less than 2 minutes.

5 tried to stand on the stick, but fell off in a few seconds

6 without any standing ability

5. raise body swing test (Elevated Body Swing Test, EBST) :

It is used to measure the motion asymmetry. When measuring EBST , the rat's tail root is first lifted by hand. The rat's head is about 5cm away from the plane . At this time, the rat's head will rotate to the left or right side. When the angle is greater than 100 , the standard is counted. The direction and angle of rotation are recorded. After one test, the rats are rested for 1 min , and then the next experiment is repeated. The test is repeated 20 times, and the total number and direction are recorded.

6. The One- Step Passive Avoidance Test (SDPAT) is a simple behavioral model for studying memory. It is mainly used to study memory consolidation and memory retention . The model contains a certain amount of spatial memory components. The test chamber is a rectangular enclosed space with a height of 60 cm . There is a small platform 9 cm X 9 cm in the box . The bottom is about 2 cm higher . The animal can only adhere to it very comfortably. On the platform, if you run down the platform ( copper rod, power, 30 V) , you will be shocked. The training process is like this : First, the animal is placed on the platform. The rat usually stays on the platform for only a few minutes and will run down. However, since the metal strip on the bottom is energized, the rat can look for a period of time. Retrieve the platform. At this point, the first test was completed. After 24 hours , the rats were placed on the platform again. At this time, the metal strip on the bottom surface was not energized. The time of the rats staying on the platform was recorded, according to the length of the test interval and the platform. The amount of residence time was used to determine the rat's ability to retain memory from the last shock.

7. The water maze test (Water Maze Test, WMT):

Used to measure the spatial learning ability of rats. Each group was performed at week 7 after transplantation . Under the same room temperature ( 25±2 ) 0C and quadrant conditions, the three groups of animals were continuously trained for 4d , 2 times / d , each time limit is 120s , more than 120s still can not find the platform, recorded as no platform found, 2 times The interval was 10 min and the test was performed on the 5th day.