Human anti-mouse immunoglobulin antibody ELISA test kit
Human anti-mouse immunoglobulin antibody ELISA kit (United States Cortez imported, number: 1027Z)
Introduction to the introduction :
The human anti-mouse immunoglobulin antibody is also called HAMA. Cortez's enzyme-free test kit enables highly sensitive detection of HAMA. The complete reagent composition (including standard and positive control) of this ELISA test kit is ready to use. Type. The concentration of the diluted sample can be directly read from the standard curve.
Add the sample and enzyme-labeled mouse IgG to the 96-well microplate, and then add the original substrate solution to develop color. Finally, add the stop solution to stop the enzyme reaction, and read the absorbance at 450 nm.
The test should be completed within 1 hour.
provide materials
1 microporous reaction plate: 8x12 strips, pre-coated with mouse IgG, 96 wells
2 Enzymes (11 mL): Horseradish peroxidase-labeled mouse IgG
3 sample diluent or zero standard (50 mL)
4 Reference Standards (0.5 mL per vial): Concentration: 25, 100, 400, and 1000 ng/mL BSA Buffer
5 HAMA positive property control (0.5 mL)
6 concentrated washing solution (20x) (50 mL)
7 Reagent A (11 mL): Hydrogen Peroxide Buffer
8 Reagent B (11 mL): TMB Buffer
9 Stop solution: 2N HCl
10 plate holder: for fixing microplate
11 User's Guide
** Except reagents A and B, the remaining reagents contain 0.01% thimerosal as a preservative
Required for experiments but not provided with equipment <br> Microplate reader with one-time filling nozzle and 25L uL, 50 uL and 100 uL pipettes
The HAMA concentration of each sample was read from the standard curve, and the final concentration result was multiplied by the dilution factor (101). The concentration higher than the highest standard was further diluted and retested. Record each sample ug HAMA/mL.
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