Introduction of sterilization methods for edible fungi

The medium is sterilized by physical methods and chemical methods. Among them, the more commonly used, and the effect is good, and the effect is stable by the physical method of heat sterilization. Several common methods are now introduced.
First, high-pressure steam sterilization: requires a high-pressure steam cooker, technical indicators zui high temperature up to 140 degrees Celsius or 2 kg / cm 2 pressure. The article is in a closed container, and the water is heated to generate high-pressure steam to raise the temperature for sterilization purposes. The general medium can be sterilized by holding the 121 degree Celsius for 20 minutes, and the mycelium trophozoites, spores, and spores have all been killed. However, if the matrix contains glucose, horses, bean sprouts, vitamins and other substances, it is better to use 115 degrees Celsius for 20 minutes, otherwise too high temperature will destroy nutrients, and the production of toxic substances is not conducive to the growth of edible fungi. Wood chips, bagasse, cottonseed hulls, wheat grains, forages, manure and other substances originally contain a large amount of bacteria and a small amount of water, and should be sterilized by using 129 degrees Celsius for 12.5 hours.
Second, atmospheric pressure steam sterilization: the items are sterilized in the pot with atmospheric steam. Usually the steam temperature is 95105 degrees Celsius, so the sterilization time requires 68 hours to achieve the effect. This method is suitable for manpower, fuel is sufficient, and there is no place to purchase a high pressure steamer. This method is better for maintaining nutrients in the medium.
Third, the intermittent sterilization method: for items that should not exceed 100 degrees Celsius high temperature is better. The medium was placed in a pot and steamed at a temperature of 100 ° C for 1 hour to kill the vegetative body of the bacteria, and the temperature was kept at 2530 degrees Celsius for 24 hours, and the remaining spores were germinated into vegetative bodies. A second cooking was carried out for 1 hour to kill the germinated spores. The same method, the third cooking for 1 hour, insulation, etc., can achieve the effect of complete sterilization.
4. Filtration and sterilization: Some materials that are not resistant to high temperature are removed by mechanical retention techniques. Such as serum, vitamins, amino acids and other substances are often used to remove bacteria. It is required to set up a suction filter bottle and an air compressor.

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