Three measures for breeding high quality Pleurotus ostreatus
The species are divided into parent species, original species and cultivars. It is also commonly referred to as primary, secondary, and tertiary, and its cultivation methods vary. The technical points are now described as follows:
1. Maternal culture: refers to the species obtained by tissue separation or spore isolation. The mother medium is usually prepared using potato acacia medium. 1000 ml of medium was prepared, using 200 g of potatoes, 18-20 g of agar, and 20 g of glucose.
Practice: Wash and peel the potatoes first, cut into small pieces of broad beans, boil in 1000 ml of water for 30 minutes, filter with 6-8 layers of gauze to make up the loss of water. After the agar was added to the solution, the dissolved sugar was added to replenish the water to 1000 ml. After the test tubes are packed, each tube is loaded with 1/5-1/4, and tampon and paper are added. Sterilize for 30 minutes at a pressure of 1.2 kg and slant it into a bevel. After cooling, it can be inoculated without any bacteria after being placed at 25 ° C to 27 ° C. One mother can transfer 25-30.
The test tube inoculated under aseptic operation is cultured under the constant temperature of about 25 ° C, and the hyphae can be filled with the test tube in about half a month. Care should be taken to remove contaminated tubes from the culture process. The cultured mother seeds are transferred to the refrigerator, stored at 4 ° C for half a year, or stored at room temperature for three months.
2, the original seed culture: raw materials can be used wood chips or cottonseed. Sawdust medium formula: 78% wood chips, 20% wheat bran or rice bran, 1% sugar, 1% gypsum. Cottonseed skin medium formula: Dilute 3% of juice with 50 kg of cottonseed and potato, (1.5 kg of potato cut into mud and boil for 30 minutes), sugar 0.1%, magnesium sulfate 0.1%.
Practice: mix the culture material with water and mix it evenly. The humidity should be tightly held by hand. It is advisable to see the water drops in the finger joints. The water ratio is generally 1:1.2-1.3. Then bottling, the bottling method is to gently shake the bottle while installing, so that the upper part of the bottle is tight and moderate, and the bottle is pressed to the shoulder of the bottle to flatten the material, and the culture material inside and outside the bottle mouth is washed, and the tampon paper bundle is added. It is placed in an autoclave for sterilization and sterilized for one hour under a pressure of 1.5 kg. After cooling, inoculate under aseptic processing conditions. The mother seed in the test tube is connected to the bottle, and one mother seed can be connected to 4-5 bottles of the original seed. After inoculation, it can be cultured at a constant temperature of 25 ° C for about one month, and the hyphae can be overgrown, which is the original species.
3. Cultivation of cultivars: The preparation of cultivars is actually an expansion of the original species. The formulation of the cultivar sawdust medium is the same as the formulation of the original culture. The formula of the cottonseed skin medium is also the same as that of the original seed culture. After bottling, the original strain is inserted into the cultivar bottle under aseptic conditions. A bottle of 750 ml of the original seed can be connected to 80-100 bottles of cultivar. After inoculation, it is incubated at a constant temperature of 25 ° C for about one month. After the bottle is full, it can be sown. When cultivating the original species and cultivars, check every 2-3 days after inoculation until the hyphae are full and the surface is inspected once a week. Pick out the bacteria contaminated with bacteria and remove them from the culture room.
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