What should we pay attention to when the electrophoresis device is powered?
Introduction to common problems and solutions for electrophoresis
First, the output of the electrophoresis instrument does not reach the set value
A: The output value of the electrophoresis instrument follows the "Ohm law": voltage U = current I × (electrophoresis tank) resistance R
When the resistance R is relatively constant, any one of U, I, P (power P = current I × voltage U) is constant, and other parameters are also constant; and any one parameter changes, other parameters follow Proportional change.
If the output voltage U of the electrophoresis instrument does not reach the preset value, first observe whether I or P has been constant, or have reached the zui big I or P specified by the electrophoresis apparatus (JY electrophoresis has clear indicator marks). If the limit value has not been reached, the voltage output can be increased by increasing the setting of the constant I or P (if necessary to the limit value).
If the current I of the electrophoresis device does not reach the preset value, the voltage U or the power P can be adjusted.
If the power P of the electrophoresis device does not reach the preset value, the voltage U or current I can be adjusted.
Second, computer controlled electrophoresis instrument overvoltage alarm
(1) Check if it is used at no load. (2) Whether or not the electrophoresis tank is buffered. (3) Whether the electrophoresis tank platinum wire is broken.
Third, overcurrent protection
(1) Whether there is a short circuit in the electrophoresis tank. (2) Whether the buffer is selected incorrectly.
Fourth, leakage protection
(1) Whether liquid is splashed inside the instrument or on the output interface. (2) Is there a lot of dust falling inside the instrument?
Electrophoresis equipment common maintenance knowledge
Tools and materials required for general maintenance of electrophoresis equipment
Tools: multimeter, electric iron, cross screwdriver, slotted screwdriver, flux,
Solder, biased pliers
First, the fan stops or makes an abnormal noise:
Replace the fan
Second, the voltage and current cannot be adjusted:
Replace the potentiometer.
Third, once a fuse:
Replace the fuse.
Fourth, continuous 憋 fuse: open the chassis, (1) check diodes D11-D14, D15-D18, D7, D8
(2) Check the R45 resistor for 474 capacitors.
(3) Check the mousse tube on the radiator. If the above three types of inspections are bad, they must be replaced.
Five, no output:
(1) Check if the fuse is damaged.
(2) Open the chassis and check if the plug connected to the front panel of the front panel is disconnected.
(3) Check if the plug of the porcelain tank transformer is off or use a multimeter to check that the two ends of each group should be the passage.
More Bio-rad Bole Electrophoresis View
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