Detoxification technology of edible fungi
In recent years, there have been many diseases of edible fungi, and so far there are no effective and special prevention and treatment drugs for production. Applying the detoxification technology of plants to the production of edible fungi can effectively inhibit the occurrence of diseases.
The so-called plant detoxification is to separate the tip tissue of the plant stem tip, and the isolated tissue is placed in a specific medium for cultivation, thereby obtaining a new seedling which is separated from the original virus and the pathogen. Specific to the detoxification of edible fungi, strictly speaking, the general tissue separation can not achieve the purpose of detoxification of the strain, which is because the fruiting body has grown to the middle and late stages, the probability of carrying viruses and germs is quite high, so The effect of detoxification is not obvious. The detoxification of the tip of the edible mushroom tip is ideal.
There are two specific methods for detoxification:
1. Mycelial tip detoxification technology
Use a petri dish measuring 90 mm or 110 mm. When there is no such culture dish, it is also possible to use a large test tube with a size of 25 mm and 200 mm, but the operation is not convenient.
First prepare the mother seed culture medium, put it into a triangular bottle, seal the cotton plug, and wrap it with leather paper; at the same time, wash the culture dish and wrap it in kraft paper. Both are sterilized at the same time. Sterilize at 121 ° C for 30 minutes. When the pressure in the sterilizer is zero, remove the sterilized material and put it into the pre-sterilized inoculation box. Open the kraft paper envelope, a hand-held culture dish, a hand-held triangular bottle, use the finger to adjust the lid of the culture dish to open about 1 to 2 cm, pour the medium into the liquid about 10 to 20 ml, and cover it tightly. The culture dish is placed flat and cooled to form a smooth surface, commonly known as a flat plate. When the plate is cooled to below 30 ° C, the strain to be detoxified is connected. Inoculation method: Pick a seed source of rice size and access the edge of the plate. After inoculation, it is cultured at the specified temperature (depending on the temperature of the variety). When the hyphae germinate to a maximum of 2 cm, cut the tip of the hyphae about 1 mm with a sharp-blade inoculation tool and insert it into the new plate. This is 1 time detoxification. Generally, it can be continuously detoxified 3 to 4 times. The more the number of detoxification, the more assured the detoxification effect.
2. Original tissue detoxification technology
The technology completely eliminates the disadvantages of the fruiting body in the middle and late growth stages, the possibility of carrying viruses and germs, and truly achieves the purpose of detoxification of the isolated tip tissue. The specific operation is:
The base was conventionally formulated to have a nitrogen ratio of 30:1. The carbon to nitrogen ratio should not be less than 25:1, so as to prevent the mycelium from growing excessively and forming a fungus, which is not easy to be primordial. The large canned bottle is washed and used. Prepare a few pieces of cotton gauze 11 cm 11 cm. When loading 1 cm below the mouth of the bottle, spread a layer of gauze from the mouth of the bottle and use a flat-blade screwdriver to insert the edge of the gauze under the bottle so that it fits snugly against the bottle. Sealing sterilization, inoculation, culture and the like are carried out as usual. After the hyphae are filled up, the temperature difference, wetness difference, light and other irritations are applied, and the primordium is generally displayed in about 7 days. At this time, the form of the primordium is white. When the primordium is as high as 1 cm, the detoxification separation can be performed.
After the flask is scrubbed with 75% alcohol, it is transferred to a sterilized inoculation box, and no conventional fumigation is carried out in the box. At the same time prepare the inoculation needle (or inoculation fishing), multiple blades (for alternate use), put it into the inoculation box together with the fungus bottle, spray the new cleaning to ensure aseptic operation. Both hands are scrubbed with 75% alcohol, extended into the inoculation box, and the blade is sterilized by burning and hand-cooled. Open the vial seal and use both hands to remove the primordium with sterile forceps. Since the material surface is covered with a layer of gauze, the base material is not taken out and the operation is convenient. The original surface layer was cut with a blade by about 1 mm, and then the original base was cut with a sharp-edged blade so that each piece was about 1 mm 2 . The isolated primordial block is transferred to a plate or a large test tube by an inoculating needle for routine culture. Operation points: First, when cutting and cutting with a blade, the blade must be replaced once for each knife; second, the separation block must be placed at the edge when it is connected, and can be connected to the edge of the plate or the foremost part of the test tube, generally not centered. .
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