Fertilizer Effect Test Technical Regulations in Guangdong Province (Trial)

Fertilizer Effect Test Technical Regulations in Guangdong Province (Trial)
1. Suitable for scope and basic requirements

1.1 This Regulation is formulated in accordance with the "Technical Regulations for Fertilizer Efficiency Test (Trial)" issued by the National Agricultural Technology Extension Service Center [(2000) Agricultural Technology (Fat) Letter No. 6], in conjunction with the actual situation in our province.

1.2 This regulation is applicable to the fertilizer effect appraisal of new fertilizers (including foliar fertilizers, soil conditioners, plant growth regulators, etc.), crop special fertilizers, formula fertilizers, microbial fertilizers and special functional fertilizers in Guangdong Province.

1.3 The unit entrusted with the test shall provide proof of the product's harmlessness to the environment, human beings and livestock, as well as a list of fertilizer ingredients and instructions for use of the product. The test undertaker shall keep its core technology confidential.

1.4 The unit responsible for the tests is the provincial, municipal, and county agricultural research, promotion, and teaching departments.

2. Design of test plan

According to the type of fertilizer, nutrient content, mechanism of action, the use of methods and commissioned by the test unit's views and other design of the test program.

2.1 Fertilizer Introduction and Test Purpose

Indicate the name of the fertilizer to be tested, the source of fertilizer, the type and main characteristics of the fertilizer, and the purpose and significance of the test demonstration.

2.2 Test Materials and Test Arrangements

2.2.1 Test materials: Includes fertilizers for testing and other fertilizers used in the tests.

2.2.2 Test soil: Select the province or local main soil types, such as red soil, red soil, vegetable garden soil, paddy soil and so on.

2.2.3 Trial crops: Select representative crop varieties according to the manufacturer's requirements. There should be no less than 2 test crops per product and no less than 2 test sites per crop.

2.2.4 Distribution of test points: According to the experimental site where the test crops are to be arranged, the test site should be the main planting area of ​​the test crops, taking into account the representativeness of the surface.

2.3 Test treatment

2.3.1 New Fertilizer

- New fertilizers mainly used for spraying, seed soaking, root irrigating and rooting, with three treatments, namely treatment 1 (regular fertilization + new fertilizer), treatment 2 conventional control (conventional fertilization) and treatment 3 blank control (conventional fertilization) + equivalent water).

- Solid new type fertilizer mainly used for seed dressing, seed fertilizer, basal fertilizer and top dressing, with three treatments, namely treatment 1 (conventional fertilization + small amount of fine soil + new fertilizer), treatment 2 conventional control (conventional fertilization) and treatment 3 blanks Control (regular fertilization + equal fine soil).

2.3.2 Special Fertilizer for Crops

Crop-specific fertilizers are compound fertilizers that are specifically formulated and produced for the nutritional characteristics of certain crops. There are four treatments, namely treatment 1 (application of special fertilizer provided by the plant), treatment 2 (conventional fertilization), treatment 3 (and One equal amount of N, P, K fertilizer control was treated and 4 blanks were treated (no fertilizer application).

2.3.3 Microbial Fertilizers (Fungicides)

Set four treatments, namely treatment 1 (regular fertilization + microbial fertilizer), treatment 2 [conventional fertilization + inactivated microbial fertilizer (matrix)], treatment 3 (conventional fertilization), treatment 4 blank control (without fertilization).

Method for inactivating matrices (preparation method for treatment 2 of 2.3.3): Take a representative microbiological fertilizer test sample, which is divided into two, one of which is left as treatment 1, the other is treated as inactivated and treated as 2. The inactivation treatment is generally sterilized by irradiation with a strong radioactive wire, and the place where no conditions for radiation sterilization can be used for medical high pressure and high temperature sterilization. The sterilized sample is generally sent to the microbiology laboratory for inactivation test. If the test fails, it must be reinactivated until it passes the test.

2.3.4 Special function fertilizer

The test treatment is set for the characteristics of the product, and the test design is subjected to a single factor difference test. For example, the XX long-term fertilizer is used for the trial design. For the special function "long-lasting" design test treatment, there are three treatments. That is, treatment 1 (long-term fertilizer), treatment 2 (treatment with nutrients such as treatment 1), and treatment 3 (conventional treatment).

2.3.5 Sample Inspection

Fertilizer appraisal test fertilizers should provide inspection reports provided by provincial-level and above-certified fertilizer inspection agencies. According to the list of product ingredients to be tested, special function fertilizers should also be selected according to their characteristics.

2.4 Test Area

The size of the experimental error is closely related to cell shape and cell area. When the area of ​​the plot is the same, increasing the area of ​​the plot along the direction of greater variation in soil fertility can effectively reduce the experimental error. Appropriately expanding the area of ​​the area can summarize the complexity of the soil, reduce the difference in soil fertility among the communities, and reduce the experimental error.

2.4.1 Cell Shape

The ideal shape of the community is a rectangle. However, it is not that the larger the aspect ratio is, the better, the community is too long, the marginal effect is increased, and the error increases. The aspect ratio of a cell is generally 2-5:1. When the size of a typical cell is large, the aspect ratio is 3-5:1. When the cell area is small, it is usually 2-3:1. When determining the shape of the plot, such as corn and other crop cultivation, it is necessary to ensure that the width of the plot can leave enough production lines or production area.

2.4.2 area of ​​the cell

The area of ​​the Daejeon Crop Community is generally 20-40 square meters, the area of ​​vegetable crops is 5-10 square meters, and 3-4 fruit trees are one community, and 1 plant stays between the communities for protection. With less processing, the area of ​​the cell can be appropriately increased; more processing is required, and the area of ​​the cell is appropriately reduced. In the hills, mountains and slopes, the area of ​​the plot should be small; in the plain area, the area of ​​the plot can be larger.

2.4.3 Cell arrangement

The basic principle of the arrangement of field trial communities is random arrangement.

2.4.4 Zone Configuration

The design of the community is rectangular, and the orientation of the plots is perpendicular to the soil fertility direction.

2.5 Test duplication

The number of repeats of the fertilizer effect test shall not be less than 3 times.

2.6 test points

No less than 2 test sites for each crop.

3. Field operations

3.1 Test crops and test years

3.1.1 Test crops

Test Fertilizer Fertilizer Efficiency For general-purpose fertilizers, at least two or more kinds of crops are generally tested (except for crop-specific fertilizers and formula fertilizers). If it refers to the products of certain crops, at least two or three representative varieties should be included. For example, vegetables can be selected from leafy vegetables, rhizomes, and eggplants.

3.1.2 Test period

To test the fertilizer effect of a fertilizer, it is usually necessary to test it for one year or one year.

3.2 Selection and preparation of test sites

3.2.1 The test site should be representative

Select the fields that are basically consistent in terms of climatic conditions, soil types, and soil fertility. As far as possible, choose a land with flat terrain, uniform shape, and uniform soil fertility to ensure the conditions necessary for the growth and development of the test. When conducting tests on sloping land, choose a plot with a gentle slope, small differences in the upper and lower land, and good irrigation conditions. Pilots should avoid roads, compost sites, etc. in order to avoid differences in soil fertility caused by these effects.

3.2.2 Fully prepared

Select a plot with similar soil fertility, preferably the same farmer's plot, select the site for the plot, and then plan the plot according to the field plan in the pilot plan; the plot can be single-irrigated in a single row to avoid string-arrangement. The area of ​​the cell is accurate and the location of each cell is appropriate. At the same time, in order to ensure the safety and precision of the test, multiple lines of protection are set around the experimental field according to different situations.

3.3 Implementation of the test plan

According to the experimental scheme and fertilizer use methods (including base application, acupoint application, seed dressing, root picking, spraying, and top dressing, etc.), field fertilization should be conducted under the same conditions as far as possible.

3.4 Field Management and Observation

3.4.1 Field Management

Following the principles of “optimality” and “unanimity”, the fertilization, sowing, irrigation, and weeding in the community must all be completed within one day. It is impossible to ensure that the management measures of one district are completed on the same day.

3.4.2 Observations

The contents of the field observations roughly include the following aspects (see Table 3):

——Basic conditions of the test site: site for test implementation, name of the test manager or farmer, terrain level, soil type, soil texture, soil fertility level, previous crop, tested crop species, planting density, etc., when conditions are available or necessary It should also provide experimental geographic properties and analysis data, and draw a plot of the community;

——Cultivation and management: Method and time for field operations such as site preparation, sowing, transplanting, fertilizing, irrigation, drainage, cultivator, and pest control;

- Investigations on fertility, fertility traits and yields: field surveys were carried out according to the items in the survey forms;

- Natural environmental factors.

3.5 Harvesting and Production

Yield is the most important indicator to judge the effectiveness of fertilizers. Harvesting and production must be done without errors and reflect the test results correctly.

——When the experiment is harvested, it is required that each cell should be singles, singles, and single production. It is impossible to mix several identically treated cells and lose the meaning of repetition.

- When harvesting, protect the plants and take them away. General grain, threshing, drying and weighing after weighing, can also be weighed out of the net weight after bad weather, mix and take 1 kg dry to calculate its drying rate. Harvesting roots and tubers of sweet potatoes, potatoes, etc., can be weighed as they are received, but the soil should be removed. If the soil is wet, it can be dried for several hours before weighing.

——Tomatoes, cucumbers, watermelons and other melons and fruit crops need to be harvested several times. The yield of each plot at the time of harvest should be recorded separately, and the harvest time should be indicated.

- Indoor test samples should be taken prior to harvest as required, labelled, cell number, processing name, sampling date, etc.

4. Test data analysis and fertilizer effect evaluation

4.1 Analysis of test data

- Analyze and compare survey data on fertility and fertility traits. Analyze the different effects of different treatments on fertility and reproductive traits, and eliminate the rankings.

- Statistical analysis of the results of the production of the community. T test was used for the two treatments to determine whether the difference between the two treatments was significant. Three (or more) treatments were analyzed by variance. If the differences were significant, multiple comparisons were performed using the new complex-difference method (LSR method) to indicate The significance of differences between treatments.

--Economic Benefit Analysis. Calculate the input cost and output value based on the prices of agricultural products at the time, fertilizer prices, test materials and labor input, and yield increase results, and conduct economic benefit analysis.

4.2 Fertility Evaluation

4.2.1 Fertility evaluation method: It is conducted by comprehensive comparison between test treatment of test fertilizers and blank control and conventional control.

4.2.2 Fertility evaluation indicators include: effects on crop growth period, impact on agronomic traits, impact on yield and quality, but also on the soil environment and economic benefits. Among them, production, quality and economic benefits are the main indicators of assessment.

4.2.3 Compared with the blank treatment, the test fertilizers were treated with an average yield increase of 5% or more. The number of test points for which the yield increased by more than 5% accounted for more than two-thirds of the total number of test points. The total number of test points for the test sites with significant yield increase reached the total number of test points. More than one-third of them have the effect of improving crop quality and soil improvement, and are products that can be promoted for use.

4.2.4 Microbial fertilizers are required to assess the role of microorganisms, but also to assess the role of the matrix in fertilizers.

5. Writing of the test report

The contents of the test report mainly include: test source and test purpose, time and place of test execution, soil conditions of test site, climatic conditions, conditions and levels of agricultural production, test plan and test treatment, test management, test raw data and analysis results, and tests Statistical analysis and results of the data, conclusions and recommendations, test execution units, test host and title, report completion time, and affixed with official seal.

5.1 Test Report Format: Prepared using a scientific paper format.

5.1.1 Materials and Methods

- Test soil

- Fertilizer for testing

- Test crops

--experiment method

5.1.2 Test Results and Analysis

——Effects of different treatments on crop growth period

——Effects of different treatments on crop reproductive traits

- Effect of different treatments on crop yield

—— Comparison and Analysis of Economic Benefits of Different Treatments

5.1.3 Test conclusions and recommendations

5.2 Test report requirements

The test report must be completed within 10 days after the crop is harvested, signed by the trial host, and commissioned by the trial unit in triplicate.

Schedule I

Fertilizer test basic situation table

Test fertilizer name:

Test Location:

Trial crops: Crop varieties:

Soil type: Fertility level:

Topography: Former crops:

Experimental Design: Community Distribution:

(1) Processing number:

(2) Repeat number:

(3) Residential area:

Fieldwork content:

Fertilization level

Fertilizer type

Variety

Effective nutrient content

Fertilizer amount

Application method

Test bearer: Date:

Schedule II

Soil Analysis Projects and Indicators

category

project

Physical properties

Bulk density (g/cm3), total porosity (%)

Chemical properties

Organic matter (%), total nitrogen (%), alkaline dissolved nitrogen (PPM), available phosphorus (PPM), available potassium (PPM), pH, trace elements (PPM): Cu, Fe, Zn, Mn, Mo, B Wait

Schedule III

Field trials of different crops

Table 1 Rice

Table 1-1 Survey of Rice Seedlings

deal with

Basic seedlings

(million/mu)

The highest seedling

(million/mu)

Effective delivery rate (%)

Plant height (cm)

Stem base (cm)

The number of

(/plant)

Number of white roots (plants/plant)

100 dry weights on the ground (g)

Bacterial wilt (%),

I

II

III

Table 1-2 Survey of Rice Growth Period Unit: Month, Day

Birth period

deal with

Sowing period

Transplanting period

Return to the green period

Childbirth period

Jointing period

Heading period

Mature period

I

II

III

Table 1-3 Survey of Main Agronomic Traits in Rice

Agronomic traits

deal with

Plant height

(cm)

Mu effective

Number of spikes

(million/mu)

Spike length (cm)

Spike rate (%)

Total grains per panicle (a)

Setting rate

(%)

Thousand grain weight

(g)

Theoretical production (kg/mu)

I

II

III

Table 1-4 Rice Yield Survey

deal with

Community output (kg)

Community average

Yield (kg)

Mu yield

(kg)

Mu increase production

(kg)

Increase productivity

(%)

1

2

3

I

II

III

Note: 1. Plant height, number of roots, and stem diameter are average of 20 plants.

2. Incidence rate is calculated as a percent of the area of ​​incidence.

Table 2 Corn

Table 2-1 Survey of Maize Growth Period Unit: Month, Day

Birth period

deal with

Sowing period

Emergence period

Jointing period

Tasseling period

Filling period

Mature period

I

II

III

Table 2-2 Survey of Maize Fertility Traits

Fertility traits

deal with

Plant height (cm)

Leaf color

Bald length (cm)

Number of spikes

()

Grain number

()

100 grain weight

(g)

I

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