Single cell algae cultivation method (2)
2019-07-27 16:03:11
Third, inoculation
The culture medium should be inoculated immediately after it is properly prepared. Inoculation is to insert the algae selected as algae into the newly prepared medium. 1. The quality of algae species generally requires the selection of algae cultures that are inviolable, biologically resistant, and vigorously growing. The appearance of the algal fluid should be normal in color, free of large amounts of precipitates, and without obvious adherence to the wall. 2. The number of algae species is in algae species cultivated in Erlenmeyer flasks and fine glass bottles. The ratio of inoculated algae fluid volume to the amount of freshly cultured medium is 1:2 to 1:3. Generally, one bottle of algae species can be connected to three bottles to four. bottle. Relay culture and mass production culture are generally suitable for culturing in a ratio of 1:10 to 1:20. The capacity of the culture tank is large, and the method of adding the culture medium by fractionation may be adopted. The first culture water amount is about 60% of the total water amount. After a few days of culture, the algal cells have been propagated to a larger density, and 40% of the culture fluid may be further added. Continue to train. 3. Inoculation time In general, the time of inoculation is preferably from 8 to 10 o'clock in the morning, and it should not be vaccinated at night. In the morning, inoculated algae cells that can absorb uptake and exercise can be used as algae species, and the algae cells that precipitated at the bottom can be discarded and play an important role.
Fourth, training
1. Routine management tasks (1) Stirring and aerating In the cultivation of unicellular algae, it is necessary to stir or inflate. Shake and stir at least 3 times a day, regularly, every half a minute. During the cultivation process, air is usually introduced, and it can be inflated or intermittently inflated throughout the day. (2) Adjusting the light In general indoor culture, the diffuse light of the near window can be used as much as possible to prevent the strong light from being directly shot. When the light is too strong, the bamboo curtain or the cloth curtain can be used for light adjustment. Outdoor culture pools should generally have a shed-style white canvas awning to adjust the light. Insufficient illumination during rainy days can be supplemented with artificial light sources for a short period of time. (3) Adjusting temperature During the summer of culture, attention should be paid to ventilation and cooling. In the winter, indoor heating should be adopted in indoor areas such as plumbing and gas heating to increase the room temperature, and the temperature difference between day and night should also be prevented. (4) Pay attention to changes in pH In the course of cultivation, measuring changes in the pH of algae, mastering its changing rules, and taking measures to prevent overshooting are very worthy of attention. If the pH is too high or too low, it can be adjusted with hydrochloric acid or sodium hydroxide. (5) During the evening of insect and rain prevention, outdoor open-culture containers should be covered with screens or cloth covers to prevent mosquitoes from entering the culture containers to lay eggs. In the morning, the cover should be opened. Large culture ponds cannot be capped. In the morning, black rice-like mosquito eggs floating on the water and other invading insects can be fished off with small nets. When it rains, prevent rainwater from flowing into the culture pool. When blowing strong winds, as much as possible, avoid blowing large amounts of dust and debris into the culture tank.
2. Observe and check the growth conditions of algae cultivation In the daily cultivation work, a full observation must be performed at regular intervals every morning and afternoon. When necessary, microscopic examinations can be performed to grasp the growth of algae.
The culture medium should be inoculated immediately after it is properly prepared. Inoculation is to insert the algae selected as algae into the newly prepared medium. 1. The quality of algae species generally requires the selection of algae cultures that are inviolable, biologically resistant, and vigorously growing. The appearance of the algal fluid should be normal in color, free of large amounts of precipitates, and without obvious adherence to the wall. 2. The number of algae species is in algae species cultivated in Erlenmeyer flasks and fine glass bottles. The ratio of inoculated algae fluid volume to the amount of freshly cultured medium is 1:2 to 1:3. Generally, one bottle of algae species can be connected to three bottles to four. bottle. Relay culture and mass production culture are generally suitable for culturing in a ratio of 1:10 to 1:20. The capacity of the culture tank is large, and the method of adding the culture medium by fractionation may be adopted. The first culture water amount is about 60% of the total water amount. After a few days of culture, the algal cells have been propagated to a larger density, and 40% of the culture fluid may be further added. Continue to train. 3. Inoculation time In general, the time of inoculation is preferably from 8 to 10 o'clock in the morning, and it should not be vaccinated at night. In the morning, inoculated algae cells that can absorb uptake and exercise can be used as algae species, and the algae cells that precipitated at the bottom can be discarded and play an important role.
Fourth, training
1. Routine management tasks (1) Stirring and aerating In the cultivation of unicellular algae, it is necessary to stir or inflate. Shake and stir at least 3 times a day, regularly, every half a minute. During the cultivation process, air is usually introduced, and it can be inflated or intermittently inflated throughout the day. (2) Adjusting the light In general indoor culture, the diffuse light of the near window can be used as much as possible to prevent the strong light from being directly shot. When the light is too strong, the bamboo curtain or the cloth curtain can be used for light adjustment. Outdoor culture pools should generally have a shed-style white canvas awning to adjust the light. Insufficient illumination during rainy days can be supplemented with artificial light sources for a short period of time. (3) Adjusting temperature During the summer of culture, attention should be paid to ventilation and cooling. In the winter, indoor heating should be adopted in indoor areas such as plumbing and gas heating to increase the room temperature, and the temperature difference between day and night should also be prevented. (4) Pay attention to changes in pH In the course of cultivation, measuring changes in the pH of algae, mastering its changing rules, and taking measures to prevent overshooting are very worthy of attention. If the pH is too high or too low, it can be adjusted with hydrochloric acid or sodium hydroxide. (5) During the evening of insect and rain prevention, outdoor open-culture containers should be covered with screens or cloth covers to prevent mosquitoes from entering the culture containers to lay eggs. In the morning, the cover should be opened. Large culture ponds cannot be capped. In the morning, black rice-like mosquito eggs floating on the water and other invading insects can be fished off with small nets. When it rains, prevent rainwater from flowing into the culture pool. When blowing strong winds, as much as possible, avoid blowing large amounts of dust and debris into the culture tank.
2. Observe and check the growth conditions of algae cultivation In the daily cultivation work, a full observation must be performed at regular intervals every morning and afternoon. When necessary, microscopic examinations can be performed to grasp the growth of algae.
Tattoo T-Shirt is just a arm t-shirt which printed with tattoo pattern or tattoo designs. It is a fake tattoo for people who don't really want to do tattoo on their body.
Professional Tattoo T-Shirt Manufacturer in China. Specialize In Manufacturing Wholesale Tattoo Sleeve T-Shirt, Skin Tattoo T-Shirt, Tattoo sleeveless T-shirt, etc products. welcome to come to the consultation.
Tattoo T-Shirt
Tattoo T-Shirt, Tattoo Sleeve T-Shirt, Skin Tattoo T-Shirt, Tattoo sleeveless T-shirt
Redtop Tattoo Supply Co., Limited , http://www.redtop-tattoo.com