The basic purchasing principle of gel imaging system (2)

How to choose a good imaging device from a variety of markets? Are many of the devices known as - can really score full marks? It is the wish of the experimenter to have a good, stable device. The following article introduces you to the "Four Basic Purchasing Principles" for selecting an imaging system.

Principle 3:

"Only compare, no cost" We advocate practice, however, reality often does not match us, or there is no Demo machine, or sometimes we really have difficulty distinguishing performance differences based on experimental results, this time The three principles will be crucial. When we can't distinguish the performance of the instrument after checking the demo of the instrument, in addition to knowing the usage and evaluation of other people, we can only open the instrument to see the parameters. Of course, there are tricks to see the parameters. Many of the parameters of the imager have been written in dozens, but in fact, the key is just a few. When we compare the parameters of different manufacturers, we must pay attention to only the key, and those parameters that are irrelevant can be turned a blind eye. In addition, don't be deceived by the manufacturer's own rating of the corresponding product. His scoring system is only relative to his own product, even if he gives a good indicator of *5 points for high scores, and other manufacturers' products. Compared to the performance, it may still be garbage-level. For scanning imaging, sensitivity is generally not a problem. We should focus on the resolution. Of course, the resolution is as long as we can meet our requirements. For small gels, membranes and microplates, the general resolution is 25um. The left and right should be enough. After meeting the resolution, a more important parameter is in front of us, that is, the dynamic range. In short, the dynamic range determines whether you can see both strong and weak signals on a single glue at the same time. And they maintain a good quantitative relationship between them. The general dynamic range is expressed in orders of magnitude. The larger the number, the wider the dynamic range. For laser scanning imaging systems, the situation is more complicated. There are many things to consider and compare from lasers and filters to beamsplitters and detectors. The following mainly introduces the parameters of the cohesive imager commonly used in the laboratory*. According to principle 1, if you mainly use it to shoot ordinary nucleic acid glue or protein glue, then almost all the imagers on the market can meet your needs very well. In addition to the price factor, the comparison is Some irrelevant indicators such as "whether the operation is simple and the shape is fashionable". The real need to look at the possibilities is to prepare users for chemiluminescence, they have high sensitivity requirements, but also require a wide dynamic range. To capture the faint chemiluminescence, you need a good CCD camera and lens. In general, the cooling temperature of a CCD camera is closely related to the background noise. The lower the temperature, the lower the noise. Therefore, the excellent cooling temperature of -25 °C is a requirement for the camera (lower temperature, the noise reduction effect is not obvious, and the quantum efficiency is greatly affected); in addition, the larger pixels can provide higher Light harvesting efficiency; so for the same size of CCD chip, you need to pay attention to the size of the pixel. The parameters of the lens are simple. Since we only need to observe the close-range samples and generally adjust the sample position (some manufacturers even provide electric sample lifting platforms), there is basically no need to choose long lenses or zoom lenses; however, because we need to detect weak The chemiluminescence, the aperture of the lens is very important, generally the smaller the F value, the greater the amount of light passing through, and the inverse square relationship, so we generally need to choose the lens with the smallest aperture F value. In addition, if the lens is electric, we can save the trouble of opening the case and repeatedly adjusting the aperture and focus manually. Other things we need to consider include light sources, filters, and black boxes. The type of light source and the uniformity of illumination, the number of filters and the shading effect of the black box are all within our consideration. Of course, if the imager's CCD and lens configuration are good, generally these components will not be too bad.

Principle 4:

“Additional value is very important.” Many users think that only one instrument is used in the lab after buying it. In fact, the truth is far from this. Buying instruments is for use, and whether it can be used well, in addition to our own ingenuity and hard work, the technical support of the manufacturers is essential. Therefore, to buy the original goods has become the consensus of many users, because this can enjoy the on-site technical support and guidance.

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