What are the conditions and methods of application of Rhizobium?

Rhizobium is a type of aerobic gram-negative bacteria that penetrates the root hairs of leguminous plants and invades the soil from the roots to form nodules. Legumes are nodule-bearing tissues that provide the energy and mineral nutrients necessary for life and nitrogen fixation; this is the symbiotic nitrogen fixation of Rhizobium. Because all kinds of rhizobia generally have their own corresponding leguminous plants, the specificity of the rhizobial bacteria is expressed, and certain rhizobial bacteria can only fix nitrogen on certain plants. However, this kind of obligate infection is not absolute. Cross-infection between them and even non-legume-like infections still exist. This is more significant for the use of rhizobia.
At present, Rhizobium is used as a fertilizer in a large area, but also in legumes. Other crops are still in the research stage. In regions where no vetch was ever grown in the past, root nodule inoculation did not produce nodule, and fresh grass yield was extremely low. In fields where some legumes have been planted, inoculation with strong nodules also increases yield. According to data from Zhejiang, Jiangxi, and Hunan, nodule inoculation can increase the yield of green manure by 10-100% and legume seeds by 10-20%.
At present, China's rhizobial fungicides require 300 million live bacteria per gram of bacteria, and the content of miscellaneous bacteria does not exceed 1%. Generally, seeds of 6-10 acres per kilogram can be mixed. When the seedlings are grown in shade, the seed dressing is done the same day. The seed dressing method can be directly used for seed dressing or mixing fertilizer sowing, direct method such as peanut rhizobium fertilizer, 100 grams of bacteria can be added with cold water, mix well, sowing the same day. For example, if it is mixed with wolfberry fruit, the seeds can be soaked in water for 6-12 hours, then dried in a basket, then transferred to a paste, and sprinkled on the seeds spread on the ground. Can sow, but also can paste the paste of the agent with a clean spray spray, dry and sowing. Fertilizer cover species, that is, after the bacteria agent watered and sprayed on the soil for cover fertilizer.
In order to increase the rhizobia yield increase, pay attention to the following issues:
1. Choose a highly efficient symbiotic nitrogen fixation combination. When breeding high-efficiency nitrogen-fixing strains, affinity and nodulation must be measured.
2. Strictly control the production quality of good fungus fertilizer. Ensure that the fungicide has enough nitrogen content, control the impurity content, the water content is controlled below 30%, and it is stored at room temperature. The effective period will be deteriorated within three months.
3. Master vaccination techniques. According to the requirement of inoculating one mu of land for every 100 grams, the reference standard proposed by the American Rhizobium can be achieved, that is, 103-105 inoculums per small seed. 106-108 grains per grain. In the old districts where legumes are grown, some more doses will be added to ensure the advantages of inoculation. According to the cultivation conditions in different regions, appropriate addition of calcium, magnesium phosphate, calcium carbonate or boron, molybdenum and other elements, preferably before and after the application of bacterial fertilizer, will help improve the survival rate of the bacteria and seed germination rate.
4. Control soil moisture according to species. According to the test, the main and lateral roots of the bacteria are generally the highest in the next 10 days, so during this period of time, the soil water content in the field is required to be 40-80% of the water in order to facilitate Rhizobium infection.
5. Strengthen management. Symbiotic nitrogen fixation in the growth of Leguminosae and Rhizobium.

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