Gel imaging system common problem analysis solution

1, the principle of gel imaging system?
The mobility of the sample on the electrophoresis gel or other carrier is not the same. A qualitative analysis of the unknown sample is performed by comparison with a standard or other alternative standard. This is the basis for the qualitative analysis of image analysis systems. According to the location of the unknown sample in the map, it can be qualitatively analyzed to determine its composition and properties.

The sample partially absorbs the projected or reflected light, so that the optical density of the sample strip on the image obtained by the photographing is different. The optical density is linear with respect to the concentration or mass of the sample. Depending on the optical density of the unknown sample, the concentration or mass of the unknown sample can be obtained by comparing the optical density of the sample strips of known concentration. This is the basis for the quantitative analysis of image analysis systems. The use of the latest technology of ultraviolet transmission light source and white light transmission source to make the light distribution more uniform, to minimize the impact of uneven optical density on the results.

2. What is the scope of gel imaging?
1. The separation and purification results of other biomolecules such as proteins, nucleic acids, peptides, amino acids and polyamino acids are qualitatively analyzed.
2. The molecular weight of the biomolecule can be determined.
3. Can be applied to the quantitative analysis of biomolecules.

3 , the general operation steps of gel imaging?
1. Turn on the gel imaging system switch.
2. Turn on the computer and the system automatically turns on and enters the imaging software.
3. Open the front panel of the gel imaging system and choose to use the UV or white light transmission source to place the corresponding light source in place.
4. Place the sample on the sample stage of the transmitted light source.
5. Select the Upper white light source in the imaging operation interface and click the green (immediate imaging) button.

4 , is the higher the pixel, the better the product?

Pixels are to be viewed for imaging devices. For example, the pixels of a digital camera and a CCD are not comparable, and the quality of the CCD itself is more important than the pixel level. The most important indicator for the same level of CCD is its size, which is the CCD size. The larger the size, the more its own value grows geometrically.

5, the main purpose ultraviolet reflective parts of the light source that?

The UV-reflecting lamp source is not widely used, and is mainly used for providing imaging services such as paper chromatography for non-transparent materials. Because the more commonly used transparent agarose gel and polyacrylamide gel, the transmission source can be fully satisfied, so the UV-reflecting light source as an option is not included in the standard.

6. Why automatic zoom lens, what is the significance?

The automatic zoom lens eliminates the need for manual adjustment and is highly versatile, while also reducing the potential for EB contamination. In addition, it makes the CCD built-in possible, thus achieving the purpose of preventing dust, dust, and shock, and better protecting the core components to extend the service life of the product.

7, how to look at some of the main technical parameters of CCD?

The main technical parameters of CCD are CCD size, effective pixels, signal to noise ratio, and number of bits collected. Among them, CCD size and signal-to-noise ratio are the key factors affecting imaging. The larger the size, the more signals can be accepted. The lower the thermal noise, the higher the signal-to-noise ratio and the better the image quality. The number of collected bits refers to the expression range of the color gray scale. If the number of collected bits is 8, it means that there are 2 8th power gray scales to restore the color.

8, UV damage to the human body, whether protective measures?

UV has certain damage to the human body, especially the eyes. The TOCAN gel imaging system is equipped with UV protection. When the UV lamp is turned on, if the black box is accidentally opened, the UV lamp will automatically extinguish to prevent damage to the human body.

9, can directly cut glue on the gel imaging?

TOCAN gel imaging can, if you need to directly cut the glue, first press the observation window in the middle of the system, open the observation window with imported anti-UV glass, and then open the small door designed for the tapping on the left and right sides, you can cut the glue. operating.

10 , how to adjust the focus focus aperture to get high quality pictures?

The three most important tunable parameters are focal focus and aperture. Among them, the focus adjusts the sharpness, the focus adjusts the image size, and the aperture adjusts the brightness. Generally, the aperture is adjusted to a suitable brightness, then the focus is adjusted to maximize the image, and finally the focal length is adjusted. In the case of a large image, the focal length is relatively easy to adjust to the clearest state, and then the image is scaled to an appropriate size for imaging. Just fine.