Introduction to Wavelength Accuracy of UV-Vis Spectrophotometer
2022-12-25 12:05:24
Wavelength repeatability refers to the difference between the actual measured value of the wavelength and the theoretical value. It is an important technical indicator of the spectrophotometer. Especially when comparing the test results of multiple instruments, the wavelength is particularly important, if the wavelength accuracy of the instrument is not Ok, you can't compare or compare the correct results. Because the same substance, when tested at different wavelengths, will have different sensitivities due to different molar absorption coefficients at different wavelengths. Even for the same sample, the test data will not be the same. At present, many domestic manufacturers use yttrium oxide glass or yttrium solution to detect eleven points of the instrument's multiple wavelength points (241nm, 279.4nm, 333.7nm, 360.9nm, 418.7nm, 536.2). However, there are many ways to detect the wavelength accuracy of an instrument, and will not be described in detail here. The inaccuracy caused by the wavelength mainly includes: the deformation of the instrument itself, which has an influence on the transmission structure of the grating, thereby affecting the accuracy of the wavelength; the accuracy of the grating transmission structure, which has a decisive effect on the wavelength accuracy, most of the current domestic The screw rod, the sector gear and the motor are directly driven, etc., wherein the screw structure can basically achieve the wavelength accuracy of ±0.5 nm, and the motor directly drives the structure to generally achieve ±1.5 nm, and the sector gear structure can generally achieve ± 2nm, so the screw structure is relatively good. At present, there are a few manufacturers that use the sector gear and the screw to drive. Of course, the wavelength accuracy of this structure will be higher. Of course, there are other factors that can directly affect the wavelength accuracy of the instrument, such as not re-identifying for many years, the use of the motor is not good, etc. will affect the wavelength accuracy.
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