ELISPOT (Enzyme-Linked Immunosorbent SPOT) is a solid-phase enzyme-linked immunospot technique for detecting specific antibody-secreting cells and cytokine-secreting cells in vitro. It is a combination of ELISA technology and cell culture technology. New technology. A new breakthrough in the study of in vitro detection of various cell-producing cytokines and cell-secreting antibodies
ELISPOT technology advantages:
1. High specificity and sensitivity: A cell secreting this protein can be detected from 200,000 to 300,000 cells.
2. Color reaction: visible and discernible spots can be counted directly under the microscope or by the ELISPOT analysis system, and 1 spot represents 1 cell, thereby calculating the frequency of cells secreting the protein.
3. Some studies not only measure the amount of cytokine production, but also the frequency of cells that secrete this cytokine.
4. The basic method is similar to ELISA.
ELISPOT technology application:
Vaccine research
2. Autoimmune disease research
3. Cancer research
4. Prediction of rejection in transplantation
5. Infection and immune response studies
6. Epitope mapping analysis
7. Screening of immunological responses to compounds and drugs
The basic principle of ELISPOT technology
Coated Captured Ab in the plates
2. Added stimulated or unstimulated cells in the plates
3. Cytokines or Ab bound to plates
4. Detected Ab with enzyme
5. Counted spots with automated ELISpot reader systems or manually, using a stereomicroscope
References
Czerkinsky et al., A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods 1983; 65: 109.
Asai et al., Evaluation of the modified ELISPOT assay for gamma interferon production in cancer patients receiving antitumor vaccines . Clin Diagn Lab Immunol 2000: 7: 145.
Vasan et al., Phase 1 Safety and Immunogenicity Evaluation of ADMVA, a Multigenic, Modified Vaccinia Ankara-HIV-1 B'/C Candidate Vaccine. PLoS One. 2010; 5: e8816.