Isolation and Rejuvenation of Pleurotus ostreatus

The use of tissue separation and rejuvenation of Pleurotus ostreatus strains is easy and effective, and it is suitable for general growers. The main points of its operation technology are: (1) In the mushroom bed and the fungus wall, the mushroom mushroom body with early mushroom growth, no bacteria infection, compact plant shape, rounded meat, beautiful color, and ripeness of the first tide mushroom is selected. Mushrooms; (2) Take the largest 3-4 mushroom pieces in the middle of the mushroom, wipe the surface with 70% alcohol, and place it in the inoculation box; (3) Tear the mushroom slice longitudinally and approach the mushroom piece in each split surface. Take a one-grain-size mushroom meat tissue at the edge and attach it to potato bran medium. Each mushroom piece was torn 10 cracks, followed by 10 test tubes, and a total of 30-40 test tubes were isolated; (4) The tubes were incubated at 15-30°C; (5) The germination was checked every day during the culture period. The mycelium was thick and white, stout, long-term normal, green, yellow, and paste-like, and the best performing isolate was transferred to wood bran medium at 15-30°C. (6) After the mycelium is full, remove the tampon in the inoculation box, seal it with wax, wrap it in the black film and preserve it in the refrigerator at 4-9°C; (7) Compare and test the original preserved species in the next production season. As a production species. Every season, it is rejuvenated, and it can gradually improve the traits of various aspects of bacteria, so that the bacteria speed up, anti-retroversive resistance increased, the quality improved significantly.

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