Pipette correct operation, maintenance and precautions

Lebe believes that many people who use pipettes for the first time always think that the operation of the pipette is very simple, but it is not. The operation of the pipette is the first step in scientific testing and is a very basic test operation, but most people are biased towards the operation of the pipette. In fact, the pipette is small, but the operation is very much. You may wish to ask yourself a few questions, you can see if there is any deviation in your operation.

1. Has there been a situation in which the liquid has been sucked into the piston during operation?

2. Do you pay attention to ensure that there is no liquid on the outer wall of the nozzle when pipetting?

3. After the pipette is used, will the pipette range be adjusted to the maximum value?

4. After using the pipette, do you put the pipette on the table?

5. Is the pipette operating the same way when moving a thick liquid and a general liquid?

6. How many pipetting methods does the pipette have?

If the above questions are unclear, then it is necessary to look at the correct operation and skills of the pipettes that Lebe has arranged for everyone.

The first is the use and maintenance of pipettes.

Set capacity   : When setting the required capacity, it should be adjusted to 1/4 turn larger than the set capacity value, and then adjusted to the set value, so that the mechanical clearance can be eliminated, so that the set value is accurate, that is, the capacity is first The knob rotates more than 1/4 turn of the target capacity value and then down to the set value.

Followed by the correct operation of aspiration:

1. Connect the appropriate nozzles;

2. Press the control button to the first gear;

3, the pipette nozzle vertically into the liquid surface 1-6mm (depending on the size of the pipette);

A pipette of 0.1-10 ul capacity enters the liquid surface 1-2 mm

2-200ul capacity pipette into the liquid surface 2-3mm

1-5ml capacity pipette into the liquid surface 3 -6mm

Note: In order to ensure accurate measurement, the nozzle can be pre-washed 3 times, that is, the liquid is repeatedly sucked and discharged 3 times.

4. Slowly slide the control button back into place;

5, the pipette can be slightly waiting for 1-3 seconds before moving out (depending on the size of the pipette);

Pause for 1 second below 1000ul

5-10ml pause for 2-3 seconds

6. Slowly remove the nozzle to ensure that there is no liquid on the outer wall of the nozzle.

Drain operation   :

(1) The nozzle is pressed against the inner wall of the capacity at an angle;

(2) Slowly press the control button to the first gear and wait for about 1-3 seconds;

(3) During the process of pressing the control button to the second gear, the suction nozzle drains the remaining liquid;

(4) Slow release control button;

(5) Press the ejection button to project the nozzle.

In the "Proper Operation of Pipettes" above, Lebe has already compiled knowledge about the correct operation of pipettes. In the following, Lebe will continue to organize the maintenance and related precautions for pipettes.

First let's take a look at how the pipette should be cured :

1. When the liquid accidentally enters the piston chamber, we should remove the pollutants in time;

2. After the pipette is used, we need to adjust the pipette range to the maximum value and place the pipette vertically on the pipette holder;

3, according to the frequency of use, all pipettes should be regularly washed with soapy water or 60% isopropyl alcohol, then washed with double distilled water and dried;

4. Avoid placing the pipette at a higher temperature to prevent deformation or leakage or inaccuracy;

5. Find problems and find professionals in time.

☆ Precautions

1. When the pipette nozzle has liquid, do not place the pipette horizontally or upside down to prevent liquid from flowing into the piston chamber to corrode the pipette piston;

2. Correctly use pipette to absorb and drain liquid to achieve high precision. Pay particular attention to the operation of steps (2) and (3) in the draining process mentioned in the "Proper Operation of Pipettes" above;

3. Method of checking whether the pipette leaks at ordinary times

After absorbing liquid, stop in the liquid for 1-3 seconds to observe whether the liquid level in the nozzle drops. If the liquid level drops, first check if there is any problem with the tip. If there is any problem, replace the tip. After replacing the tip, the liquid level is still A drop indicates a problem with the piston assembly and should be repaired by a qualified service technician.

4. Pipettes that require high temperature disinfection should first check whether the pipette used is suitable for high temperature disinfection before processing.

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