The world's first "use" goat cloned Asian yellow sheep

Professor Chen Dayuan of the Institute of Zoology, Chinese Academy of Sciences, revealed here on the 24th that the world’s first somatic cell clone, the Asian Yellow Goat, was successfully established in Shandong. This is the first time in the world that "use" goats to clone Asian yellow sheep. The Asian Yellow Sheep, also known as Nanjiang Huangyang, is a new breed of meat goat that has been successfully bred by animal science and technology personnel in China after 40 years of meticulous selection and is the only country in China that has passed the national appraisal and is named by the country. The breeding and rapid breeding studies are listed as “863”. "The plan and the national high-tech industry development plan. In close cooperation with Shandong Shengneng Group, the researchers boldly innovated in the cloning research of Asian Yellow Sheep, using a new method of enucleation of the oocyte at the end of the day and combined with direct injection of the entire somatic cells in the cytoplasm, which greatly improved the cloning efficiency and achieved "Four highs and one low": high pregnancy rate (24%), high birth rate (20%), high survival rate (83%), high yield (100%), low abortion rate (16%). Chen Dayuan of the Institute of Zoology, Chinese Academy of Sciences, said that all cloned individuals have been verified by microsatellite analysis that their nuclear DNA is the same as the donor cell and not related to the surrogate ewes. Successful cloning of Asian Yellow Sheep is of great significance. Not only did the adult Asian yellow sheep's fibroblasts dedifferentiate and restore pluripotency in the enucleated oocyte cytoplasm of common goats, indicating that normal goat oocyte cytoplasm can “instruct” the Asian yellow sheep body. The nucleus develops, and can “guide” the reconstructed embryos of different breeds to be implanted in the recipient uterus of ordinary white goats, black goats, and dairy goats, and the whole course of pregnancy and childbirth is completed. In addition, high-efficiency animal yields provide new methods, important scientific research and technical reference data for the conservation and rescue of endangered livestock species in China and the world. The cloning of Asian Yellow Sheep data provides valuable insights into the in-depth study of cloned animals. Scientific basis and experience.

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