BCA Protein Quantitation Protocol - The cheapest BCA Protein Quantification Kit Technical Description
Accurate protein quantification is essential for protein-related experiments involving molecular biology, cell biology, biochemistry, developmental biology, and neuroscience. The most commonly used methods for quantitative protein analysis are the BCA method and the Bradford method. Based on the principle of the Bradford method, due to the different contents of arginine and aromatic amino acids in various proteins, different proteins have large deviations in measurement and are susceptible to detergents (Triton X-100, SDS...). Interference. Therefore, more authorities and customers will choose the BCA method to detect protein concentration. For example, the FDA-designated BCA kit is the only standard reagent for protein detection in new drug application materials.
Experimental principle of BCA method: Under alkaline conditions, the peptide bond structure in the protein molecule can combine with Cu2+ to form a purple complex, and at the same time reduce Cu2+ to Cu+. The BCA reagent can bind sensitively and specifically to Cu+ to form a stable colored complex. The highest absorption value at 562 nm can be measured at 540-595 nm. The color depth is proportional to the protein concentration and the standard curve. In contrast, the concentration of the protein to be tested can be calculated.
Advantages of Biovision 's conventional BCA protein quantification kit :
· The experiment is simple and convenient;
· High sensitivity, the lower limit of detection concentration reaches 25μg/ml;
· The protein concentration is not affected by chemicals such as detergents in most samples, and is compatible with SDS, Triton X-100, and Tween 20;
· There is a good linear relationship in the concentration range of 20-2000μg/ml;
· Detection of different protein molecules with a coefficient of variation less than the Bradford method protein quantification;
· Unbeatable price/performance ratio, the cost of a single experiment is lower than that of a domestic kit.
| Product name and description | Brand | Item number | product manual |
| BCA Protein Assay Kit II | Biovision | K813-5000 | Details |
Of course, the BCA method also has its shortcomings, that is, it is easy to receive interference from a slightly high concentration of reducing agents, such as DTT, TCEP and β-mercaptoethanol. To this end, as a global life sciences company leader, Biovision has thoroughly explored the shortcomings of BCA and launched the only BCA kit on the market that is compatible with high-concentration reducing agents, K818-1000. Compatible with: TCEP (20 mM), DTT (10 mM), β-ME (35 mM)
Figure 1. Excellent performance of the Biovision kit (K818-1000) under high concentration of reducing agent
| Product name and description | Brand | Item number | product manual |
| BCA Protein Assay Kit - Reducing Agent Compatible | Biovision | K818-1000 | Details |
Cables And Adapters,USB Cable,HDMI Cable,Speaker Cable,Network Cable,Type-C Cable ,Audio&Video Cable
Changzhou Satidi Import and Export Co., Ltd. , https://www.guanjiejts.com