Nature: Britain builds a pluripotent stem cell bank to reveal genetic variation
In a new study, an alliance, including the Wellcome Foundation Sanger Institute in the UK, built the UK's largest high-quality human-induced pluripotent stem cells (induced pluripotent stem cells). iPSC, iPS cell) library. This iPS cell bank contains hundreds of stem cell lines. After being fully labeled, these stem cell lines are available for independent research and are a powerful resource for scientists to study human development and disease. The relevant research results were published online May 10, 2017 in the journal Nature, entitled "Common genetic variation drives molecular heterogeneity in human iPSCs".
In collaboration with King's College London, the European Institute of Bioinformatics, the University of Dundee and the University of Cambridge, the study also explored in unprecedented detail the large variations in these stem cell lines from healthy people.
Technological advances have made it possible to obtain an adult cell and use specific growth conditions to return it to an early embryonic state. This causes iPS cells to be produced. iPS cells can become any type of cell in the body. These cells have enormous scientific potential for studying the production and effects of diseases such as cancer, Alzheimer's disease and heart disease.
However, the process of constructing iPS cells is lengthy and complex, and only a few laboratories are able to describe their cellular characteristics to make them suitable for use by other scientists.
The Human Induced Pluripotent Stem Cell Initiative (HipSci) uses standardized methods to construct iPS cells on a large scale to study differences between healthy people. A reference stem cell bank was constructed using skin biopsies donated by 301 healthy volunteers, and a variety of stem cell lines from each human were constructed.
These researchers constructed 711 stem cell lines and obtained detailed information about their genome, the proteins they expressed, and the cell biology characteristics of each stem cell line. Academic researchers and industry personnel are able to obtain stem cell lines and data generated by HipSci.
This plan took four years to complete. It requires researchers from many different disciplines to work together to adopt an interdisciplinary approach.
Dr. Daniel Gaffney, Ph.D., co-communication author and researcher at the Sanger Institute at the Welkom Foundation, said, "We have developed a comprehensive library of high-quality reference human iPS cell lines from healthy volunteers. Each iPS cell line is widely available. Research, and the broader research community, can get these cell lines and their annotated data. This resource is a stepping stone for researchers to build better cell models for many diseases because they can study many cell types (including normal The risk of disease in those cells that are not available in the situation."
By constructing more than one stem cell line from each healthy person, these researchers were able to determine the similarity of stem cell lines from the same individual.
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