Extraction of pectin from watermelon rind
Pectin is rich in watermelon rind, which is a good raw material for pectin extraction.
1, raw material cleaning: use fresh, non-rotated watermelon rind, remove sediment, clean.
2, steam material: In order to kill the pectinase in living cells, the washed melon skin into the steamer, steamed until the melon skin becomes soft, there is water precipitation dripping degree.
3. Pressing: The steamed rind skin is squeezed into a cloth bag and squeezed as much as possible to remove the moisture in the tissue cells and the sugars, inorganic salts and other substances dissolved therein to increase the purity of the pectin.
4. Hydrolysis: Place the dried ingredients in a corrosion-resistant container, add 3-4 times the amount of water to the rind, add acid to adjust the pH to about 2, and heat to 98 to 100°C for a certain period of time. This step should master the acidity, temperature and time. If the acidity is large and the temperature is high, it takes a short time; otherwise, the pectin will be excessively hydrolyzed; if the acidity is small and the temperature is low, it takes a long time; otherwise, the pectin hydrolysis Not out.
5. Filtration: Use a bag to filter, collect the filtrate, and perform a second hydrolysis of the filter residue.
6, the second hydrolysis: filter residue plus 2 times the water, with an appropriate amount of acid to adjust the PH value of about 2, with the above method and then hydrolyzed and filtered once, discarded the filter residue, the two combined filtrate.
7. Decoloration: Add 0.3-0.5% of activated carbon to the filtrate, decolorize at 55-60°C for 30 minutes, and collect the filtrate.
8. Concentration: The decolored liquid is vacuum concentrated to a solid content of 8%.
9. Alcohol Analysis: Add 90% ethanol to the concentrate, using 2 times the concentrate. When ethanol is added, pectin can be observed to be flocculated and squeezed after the pectin is completely flocculated.
10. Pressing: The flocculent pectin is placed in a fine cloth bag to remove the liquid.
11. Alcohol wash: The squeezed pectin was washed with 5% ethanol and then the ethanol was squeezed.
12. Drying: The solid pectin is placed in an enamel plate and baked at a temperature of 65-75°C until the moisture content drops below 8%.
13. Crushing: The solid pectin after drying is ground and crushed in a dry environment, and the pectin finished product is obtained after passing through a 60-mesh sieve.
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