Hanks balanced salt solution (5 × HBSS, containing phenol red) instruction manual
Product introduction:
Balanced Salt Solution (BSS) is consistent with environmental conditions such as pH and osmotic pressure in cell growth state, and has the functions of maintaining osmotic pressure, controlling acid-base balance, and supplying energy and inorganic salt components necessary for cell survival and metabolism. Meet the basic needs of cell survival and maintain a certain metabolism in vitro. It is mainly composed of inorganic ions, sometimes containing sodium bicarbonate, glucose, phenol red, etc., and if necessary, HEPES can be added to maintain the balance of osmotic pressure. The balanced salt solution can be used as a base medium for the complete medium, and can also be used to dilute the concentrated amino acid and vitamin solution to prepare a complete medium.
BBS formulations are often modified, such as Hank's BBS from calcium-free or phenol red, calcium-magnesium-containing phenol red, etc.
Dulbecco's PBS is free of calcium or magnesium or calcium and magnesium. HBSS, EBSS, PBS, etc. are all salt solutions associated with weaker phosphate buffers. Common balanced salt solutions are Eargle solution, Hanks solution, phosphate buffer solution (PBS), and the like. Hanks balanced salt solution is one of the most commonly used phosphate buffer solutions, also known as Hanks' Balanced SaltSolution, Hanks'BSS, or HBSS, mainly composed of sodium chloride, potassium chloride, phosphate, sodium bicarbonate, glucose, etc. Containing Ca2+, Mg2+, phenol red, the pH is generally 7.2-7.4.
Balanced salt solution (5×, containing phenol red), containing Ca2+, Mg2+, phenol red, prepared with re-distilled ultrapure water, pH 7.4, sterilized by strict filtration, low endotoxin content, can be used for trypsin-EDTA Preparation of various non- or low-calcium-magnesium cell culture solutions such as cell digestive juice, and rinsing of tissues and cells, etc., the reagent is a 5× concentrated solution, which should be diluted to 1× before use.
product composition:
| Name Specifications | 500ml | Storage |
| Hanks balanced salt solution (5 x HBSS, containing phenol red) | 500ml | 4 ° C |
| Instructions for use 1 | 1 copy | |
Operation steps (for reference only):
1. When washing the cells during cell culture, dilute according to 5×HBSS solution: sterile water=1:4, ie take 5×
One part of HBSS solution and 4 parts of sterile water are mixed thoroughly to obtain HBSS working solution.
2. For routine experimentation (no sterility required), dilute by 5×HBSS solution: double distilled water or deionized water=1:4.
Take 1 part of 5×HBSS solution, 4 parts of double distilled water or deionized water, and mix well to obtain HBSS working solution.
3. Take the HBSS working fluid for downstream experiments.
Precautions:
1. When performing cell washing during cell culture, attention should be paid to aseptic processing to avoid contamination by microorganisms.
2, Hanks balanced salt solution (5 × HBSS, containing phenol red) due to calcium ions, especially other salt ions concentration is also very high, easy to produce a little precipitation, after this situation, usually placed in a warm bath to precipitate dissolved, If more precipitate is produced, it should be discarded.
3. The reagent is sterilized by filtration.
4. For your safety and health, please wear a lab coat and wear disposable gloves.
Validity: Valid for 3 months.
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